It’s the day of the twins’ separation and interest is buzzing around Holby City . Jayne holds what she hopes will be a low key press briefing but a journalist knows that the operation has been funded by the controversial right win fundamentalist Rev Steve Randall and she is questioned about the morality of this. Jane is confused about how they know he is involved.

Connie leaves her mystery man in bed after a romantic evening with him. She has a big day ahead of her as she is the first to operate on the conjoined twins.

Kyla is tending to Dorian Roberts, who’s still recovering from his appendicitis. Roberts is still pursuing Kyla and Maddy teases her. Roberts makes a romantic gesture of champagne and a card asking her for a drink - not knowing that she is an alcoholic and Lyla takes this that she should leave him well alone. Maddy reckons Kyla should go for it and let her hair down. Kyla’s reluctant – she’s not really in the mood to see men as her luck with them hasn’t been good so far. Maddy tells her that she needs to make a change and move onwards. Kyla knows Maddy is right.

The operation begins but doesn’t start well as they are trying to anaesthetise the twins. The full team of surgeons sitting in the staff room are watching the operation start on screen and they take a collective deep breath as the first incision is made.

The board have heard about the calamitous press briefing and they want answers.from Jayne about the details of the past few months. Why did she take money from a known child abuser and why she hide it from the board in a secret account. Jayne does a good job of explaining herself but the board aren’t cutting her any slack. Randall putting money into a dodgy ‘Holby Night care’ account looks highly suspect. The board tell her that she can’t use the money from Randall so that she must find the money from elsewhere or stop the procedure. Jayne protests, stating that the operation has already started but they say that this is her problem and she needs to fix it. No more talking to the press – they need to limit the damage and if the operation goes wrong they’ll expect her immediate resignation.

Kyla thanks Roberts for the champagne and agrees to go for a coffee with him. Meanwhile, Maddy intercepts another letter which is clearly from Abra. She puts it in a draw as the amount of letters from him piles up.

The operation enters its fourth hour and Connie and Elliot are mid-flow. They run into more problems as one of the twins’ hearts is still a lot weaker than the other and the weaker twin seems to be fading. Connie doesn’t want to give up on the weak twin and is disgusted that Elliot thinks they should let one die to save the other. Connie fights on and the other twin regains strength.

Jayne continues to be asked questions about Randall, who has tipped off the world’s press about the work he is doing at Holby to lessen the impact of the child abuse claims levelled at him. The board need her to explain the situation to the press and she tries to explain that no other person, charity or the NHS opted to pay for the operation. She needed the cash and if the devil himself had offered to pay for the treatment then she would have taken it.

Jac isn’t due to be in the operation but wants to be a part of it. She watches Faye and Joseph working in theatre together and sees that Faye is doing a great job. This is all too much for Jac, as she hates the fact that Faye is with Joseph, and she tries to do something about it – making out that Faye isn’t coping very well as the operation on the twins is too close to home for her. Jac confides in Ric about Faye and her disabled son Archie which is all news to Ric. He informs Faye that she no longer needs to play a part in the procedure. Faye is shocked but realises that Jac had something to do with this.

Michael and Ric discuss the operation on screen as they are next in to operate. They are in shock as they realise how difficult it will be to separate the livers of the twins. The surgeons are all frustrated – blaming each other – why the hell wasn’t this on the scans? It’s going to be a much difficult procedure that they had ever imagined.